| Target: |
IL-18 (Interleukin 18) |
| Binding Specificity: |
AA 37-194 |
| Reactivity: |
Rat |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This IL-18 antibody is un-conjugated |
| Application: |
Immunoprecipitation |
| Specificity: |
This antibody reacts with rat IL-18. |
| Purification: |
Protein A agarose |
| Immunogen: |
Mature rat IL-18 fusion protein corresponding to 37-194 aa. |
| Clone: |
91D8 |
| Isotype: |
IgG2b |
| Concentration: |
1.0 mg/mL |
| Buffer: |
PBS containing 50% glycerol, pH7.2. No preservative is contained. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
- 20°C |
| Storage Comment: |
Upon receipt, store (in aliqouts) at - 20°C. |
| Alternative Name: |
Interleukin-18 / IL18 |
| Synonyms: |
Igif, Il-18, IL-18, IGIF, ChIL-18, IL-1g, IL1F4, IL18, interleukin 18, Il18, IL18. |
| Background: |
Interleukin 18 (IL-18) is an 18-kDa cytokine recognized as a vital factor in the production of interferon-γ (IFN-γ) during toxic shock, sharing functional traits with IL-12. Initially synthesized as a 24-kDa precursor lacking a signal peptide, IL-18 requires cleavage to become biologically active. The enzyme IL-1 converting enzyme (ICE, Caspase-1) targets pro-IL-18 at aspartic acid in the P1 position, generating the mature, active peptide readily released from cells. Sources of IL-18 include Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells, and murine diencephalon. IFN-γ, crucially produced by activated T or NK cells, plays pivotal roles in defense against microbial pathogens by activating macrophages, enhancing NK activity, and facilitating B cell maturation, proliferation, and Ig secretion. Moreover, IFN-γ prompts the expression of MHC class I and II antigens while inhibiting osteoclast activation. IL-18 primarily targets T helper type-1 (Th1) cells, working synergistically with IL-12 to robustly induce IFN-γ production. IL-18 exhibits pleiotropic effects, including the enhancement of IFN-γ and GM-CSF production in peripheral blood mononuclear cells, Th1 cytokine production (IL-2, GM-CSF, and IFN-γ) in T cells, and the upregulation of Fas ligand expression by Th1 cells. |
