2-1-1-green-tea-extract-1

Human/Mouse/Rat TNF Apha Protein (AA 77-235) (DYKDDDDK Tag)

Product Details

Target:	TNF alpha (Tumor necrosis factor alpha (TNF-α))
Protein Type:	Recombinant
Protein Characteristics:	AA 77-235
Origin:	Human, mouse, rat
Source:	E. coli
Purification tag / Conjugate:	This TNF alpha protein is labelled with DYKDDDDK Tag.
Specificity:	TNF- binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2.
Characteristics:	ED50: 0.02 ng/mL (WEHI 164 cells). The extracellular domain of mouse tumor necrosis factor-alpha (TNF-alpha) (AA 77-235) is fused at the N-terminus to an 8 AA linker peptide and a DYKDDDDK tag.
Purity:	> 95% as determined by SDS-PAGE.
Endotoxin Level:	< 0.1 EU/µg purified protein as determined by LAL test.
Format:	Lyophilized
Reconstitution:	Soluble. Prepare a 1 mg/mL stock solution by dissolving the contents of the vial in 50 µL of sterile H₂0.
Buffer:	50 µg recombinant TNF-alpha and PBS, 1 mg/mL TNF-alpha after reconstitution.
Storage:	- 20°C
Alternative Name:	TNF alpha
Synonyms:	DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa.
Molecular Weight:	~20 kDa by SDS-PAGE

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