| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 77-233 |
| Origin: |
Human, mouse |
| Source: |
E. coli |
| Purification tag / Conjugate: |
This TNF alpha protein is labelled with His tag. |
| Specificity: |
Human TNF-alpha (aa 77-233) is fused at the C-terminus to a His-tag. |
| Purpose: |
TNF-alpha (human) (rec.) (His) |
| Characteristics: |
Protein. Human TNF-alpha (aa 77-233) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.1EU/μg purified protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in 10 mM sodium citrate buffer, 200 mM NaCl, pH6.0. Purity: >95 % (SDS-PAGE). TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-alpha is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
| Purity: |
> 95% (SDS-PAGE) |
| Endotoxin Level: |
< 0.1 EU/μg purified protein |
| Format: |
Lyophilized |
| Concentration: |
Lot specific |
| Buffer: |
Lyophilized from 0.2 μm-filtered solution in 10 mM sodium citrate buffer, 200 mM NaCl, pH6.0. |
| Handling Advice: |
After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Short term storage: + 4°C.
Long term storage: -20°C.
Use & Stability: stable for at least 1 year after receipt when stored at - 20°C. Working aliquots are stable for up to 3 months when stored at - 20°C. |
| Alternative Name: |
Tumor necrosis factor alpha (TNFa) |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
TNF-alpha, a versatile pro-inflammatory cytokine, is secreted by diverse cell types including adipocytes, activated monocytes, macrophages, B cells, T cells, and fibroblasts. It is a member of the TNF family of ligands and operates via two receptors, TNFR1 and TNFR2. TNF-alpha exhibits cytotoxic effects on numerous tumor cells and is crucial for orchestrating the immune response against bacterial infections. Moreover, it contributes to the onset of septic shock, autoimmune disorders, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-alpha, comprising a soluble 157 amino acid protein (17.4 kDa), corresponds to the C-terminal extracellular domain of the full-length transmembrane protein. |
| Molecular Weight: |
~18 kDa (SDS-PAGE) |
