| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 77-233 |
| Origin: |
Human |
| Source: |
E. coli |
| Application: |
Functional studies (Func) |
| Purpose: |
Recombinant Human Tumor Necrosis Factor α/TNFα |
| Sequence: |
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
| Characteristics: |
ED50 is less than 0.03 ng/mL as determined by the cytolysis of murine L929 cells in the presence of actinomycin D. Specific activity of 3.0 x 107 IU/mg. |
| Purity: |
> 95% as determined by SDS PAGE |
| Endotoxin Level: |
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test |
| Sterility: |
0.2 μm filtered |
| Format: |
Lyophilized |
| Reconstitution: |
It is not recommended to reconstitute to a concentration less than 100 μg/mL. |
| Buffer: |
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH7.0. |
| Handling Advice: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. |
| Storage: |
4°C, - 20°C, - 80°C |
| Storage Comment: |
Lyophilized protein should be stored at < - 20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4 - 7°C for 2 - 7 days. Aliquots of reconstituted samples are stable at < - 20°C for 3 months. |
| Alternative Name: |
Tumor necrosis factor alpha (TNFa) |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
Recombinant tumor necrosis factor α/TNF-α is a cytokine devoid of glycosylation, synthesized using recombinant DNA technology in E. coli. Comprised of three identical polypeptide chains, each containing 158 amino acids, TNF-α forms a compact, bell-shaped homotrimer. The individual subunits possess a relative molecular mass of 17,484 Daltons each. This potent lymphoid factor exhibits cytotoxic effects on a broad spectrum of tumor cells and select target cells. TNF-α, with a subunit molecular mass of 17 kDa, significantly influences growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune disorders, and responses to viral, bacterial, fungal, and parasitic infections. Beyond inducing hemorrhagic necrosis in tumors, TNF-α is implicated in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune conditions like Crohn's disease, rheumatoid arthritis, and graft-versus-host disease. |
| Molecular Weight: |
17.3 kDa |
