| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Reactivity: |
Human |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Sample Type: |
Tissue homogenate, cell culture supernatant, plasma, serum |
| Analytical Method: |
Quantitative |
| Sensitivity: |
4 pg/mL |
| Application: |
ELISA |
| Plate: |
Pre-coated |
| Characteristics: |
TNF-alpha ELISA kit (rat) (tumor necrosis factor, cachectin, TNF-alpha, tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2, TNFalpha, TNF alpha, TNF a, TNFa). |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
4°C, - 20°C |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
Tumor necrosis factor-alpha (TNF-alpha), also known as cachectin, and tumor necrosis factor beta (TNF-beta), also known as lymphotoxin, are two closely related proteins with approximately 34% amino acid sequence identity. They both bind to the same cell surface receptors and exhibit many similar biological functions. TNF-alpha and TNF-beta are crucial in the body's defense against infections and the suppression of malignant tumor growth, acting as immunostimulants and mediators of the inflammatory response. However, excessive production of TNFs has been associated with several pathological conditions, including cachexia, septic shock, and autoimmune disorders. TNF-alpha is produced by activated macrophages and various other cell types such as T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells, and certain tumor cells. In rats, the TNF-alpha cDNA encodes a type II membrane protein consisting of 235 amino acid residues. |
