2-1-1-green-tea-extract-1

Human TNF Alpha Sandwich ELISA Kit (< 1 pg/mL)

Cat. No.:	SPODRP00780
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Product Details

Target:	TNF alpha (Tumor necrosis factor alpha (TNF-α))
Binding Specificity:	AA 77-233
Reactivity:	Human
Detection Method:	Colorimetric
Method Type:	Sandwich ELISA
Detection Range:	15.6 pg/mL - 1000 pg/mL
Minimum Detection Limit:	15.6 pg/mL
Sample Type:	Cell culture supernatant, serum, plasma
Analytical Method:	Quantitative
Sensitivity:	< 1 pg/mL
Application:	ELISA
Plate:	Pre-coated
Specificity:	Expression system for standard: E. coli, V77-L233
Purpose:	Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF alpha
Immunogen:	Expression system for standard: E. coli. Immunogen sequence: V77-L233.
Handling Advice:	Avoid multiple freeze-thaw cycles.
Storage:	- 20°C, 4°C
Storage Comment:	Store at 4°C for 6 months, at - 20°C for 12 months.
Expiry Date:	12 months
Alternative Name:	TNF alpha
Synonyms:	DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa.
Background:	TNF-alpha is a protein secreted by macrophages in response to inflammation, infection, and cancer. It shares biological activities with human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta), exhibiting 30% amino acid homology. TNF-alpha, primarily produced by monocytes, can prompt endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor, thus extending its role to regulate hematopoiesis in vitro. TNF is a soluble protein that selectively damages tumor cells while sparing normal cells. Purified TNF from HL-60 leukemia cells, appearing as a 17.3-kilodalton protein, exhibits cytotoxic and cytostatic effects against various human tumor cell lines. The human TNF cDNA spans 1585 base pairs, encoding a 233-amino-acid protein. The mature protein commences at residue 77, preceded by a long leader sequence of 76 amino acids. TNF-alpha is located on human chromosome 6.

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