Human TNF Alpha IQ-ELISA Kit

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Human TNF Alpha IQ-ELISA Kit

Cat. No.: SPODRP00828
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Product Details

Target: TNF alpha (Tumor necrosis factor alpha (TNF-α))
Reactivity: Human
Detection Method: qPCR
Method Type: Sandwich ELISA
Detection Range: 0.384 pg/mL - 6000 pg/mL
Minimum Detection Limit: 0.384 pg/mL
Sample Type: Cell culture supernatant, plasma, serum
Analytical Method: Semi-quantitative
Application: ELISA
Plate: Pre-coated
Purpose: Human Immunoquantitative (PCR-Based) TNF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Characteristics: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
Handling Advice: Avoid repeated freeze-thaw cycles.
Storage: - 20°C
Storage Comment: May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at - 20°C or - 80°C (recommended at - 80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.
Expiry Date: 6 months
Alternative Name: TNF alpha
Synonyms: DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa.

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