| Target: |
IL-6 (Interleukin 6) |
| Binding Specificity: |
AA 29-212 |
| Reactivity: |
Human |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Detection Range: |
4.69 - 300 pg/mL |
| Minimum Detection Limit: |
4.69 pg/mL |
| Sample Type: |
Serum, plasma, cell culture supernatant |
| Analytical Method: |
Quantitative |
| Sensitivity: |
< 0.3 pg/mL |
| Application: |
ELISA |
| Specificity: |
Expression system for standard: E. coli
Immunogen sequence: P29-M212. |
| Purpose: |
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6 |
| Immunogen: |
Expression system for standard: E. coli. Immunogen sequence: P29-M212. |
| Handling Advice: |
Avoid multiple freeze-thaw cycles. |
| Storage: |
- 20°C, 4°C |
| Storage Comment: |
Store at 4°C for 6 months, at - 20°C for 12 months. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
The human interferon-beta 2 gene (IFNB2) yields a protein identical to B-cell stimulation factor-2 (BSF-2), hybridoma growth factor (HGF) - also known as interleukin-6 (IL-6), and hepatocyte stimulating factor (HSF). Proteins originating from this gene orchestrate the plasma protein response to tissue injury, known as the acute-phase response, and oversee the growth and differentiation of both B and T cells. IL-6, in particular, has garnered attention as a potential factor in osteoporosis due to its ability to stimulate cells of the osteoclast lineage, potentially contributing to bone loss in estrogen-deficient individuals. IL-6 plays pivotal roles in immune response regulation, hematopoiesis, and bone resorption. It not only triggers the hepatic acute-phase response but also impacts adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Excessive production of IL-6, a proinflammatory cytokine, correlates with various age-related conditions such as cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2, a novel interleukin comprising 184 amino acids, shares structural similarities with IL-6. The standard product utilized in this kit is recombinant human IL-6, comprising 184 amino acids and exhibiting a molecular mass of 20.3 kDa. |
