| Target: |
IL-18 (Interleukin 18) |
| Reactivity: |
Human |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Sample Type: |
Cell culture supernatant, plasma, serum, tissue samples |
| Analytical Method: |
Quantitative |
| Sensitivity: |
4 pg/mL |
| Application: |
ELISA |
| Plate: |
Pre-coated |
| Characteristics: |
IL-18 ELISA kit (human) (interleukin-18, IL-18, iboctadekin, interferon gamma-inducing factor, IFN-gamma-inducing factor, interleukin-1 gamma, IL-1 gamma, IL18, IGIF, IL1F4, interleukin-1 gamma, IL-1 gamma, IL1 gamma, IL-1F4). |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
4°C, - 20°C |
| Alternative Name: |
IL18 |
| Synonyms: |
Igif, Il-18, IL-18, IGIF, ChIL-18, IL-1g, IL1F4, IL18, interleukin 18, Il18, IL18. |
| Background: |
Interleukin 18 (IL-18), also recognized as interferon-gamma-inducing factor (IGIF) and IL-1gamma, is a cytokine sharing biological functions with IL-12 and bearing structural similarities to the IL-1 family of proteins. Initially isolated from liver cells, IL-18 is subsequently identified to be expressed by monocytes/macrophages, osteoblasts, and keratinocytes. The physiological conversion of pro-IL-18 to IL-18 is mediated by caspase-1 (IL-1 beta-converting enzyme). Analogous to IL-12, human IL-18 is demonstrated to enhance natural killer (NK) cell activity in peripheral blood mononuclear cell (PBMC) cultures. Additionally, human IL-18 elicits the production of IFN-gamma and GM-CSF while suppressing the secretion of IL-10 by PBMC. On enriched human T cells, IL-18 can augment Th1 cytokine production and promote cell proliferation through an IL-2-dependent pathway. In murine models, IL-18 serves as a costimulatory factor for Th1 cell activation but not Th2 cells. Furthermore, IL-18 selectively amplifies FasL-mediated cytotoxicity in Th1 cells, excluding Th0 or Th2 cells. Moreover, IL-18 induces activated B cells to generate IFN-gamma, which impedes IgE production. |
