| Target: |
IL-18 (Interleukin 18) |
| Protein Type: |
Recombinant |
| Origin: |
Human |
| Source: |
E. coli |
| Purification tag / Conjugate: |
This IL-18 protein is labelled with Biotin. |
| Sequence: |
Tyr37-Asp193 |
| Purity: |
> 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Endotoxin Level: |
Less than 1EU per μg by the LAL method. |
| Sterility: |
0.22 μm filtered |
| Format: |
Lyophilized |
| Reconstitution: |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Buffer: |
Lyophilized from 0.22 μm filtered solution in PBS (pH7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Handling Advice: |
Avoid repeated freeze-thaw cycles. |
| Storage: |
4°C, - 80°C |
| Storage Comment: |
Reconstituted protein stable at - 80°C for 12 months, 4°C for 1 week. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL18 |
| Synonyms: |
Igif, Il-18, IL-18, IGIF, ChIL-18, IL-1g, IL1F4, IL18, interleukin 18, Il18, IL18. |
| Background: |
IL-18, also known as IL18, IGIF, IL-1F4, IL-1 gamma, IL1F4, IL-1g, or Interleukin 1, was initially identified as a facilitator of IFN-γ generation from Th1 cells activated by anti-CD3, particularly in conjunction with IL-12. Following stimulation with antigen and IL-12, naïve T cells differentiate into IL-18 receptor (IL-18R) bearing Th1 cells, which elevate IFN-γ synthesis upon exposure to IL-18 stimulation. |
| Molecular Weight: |
18.2 kDa same as Tris-Bis PAGE result. |
