| Target: |
IL-12 (Interleukin 12) |
| Reactivity: |
Human |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Detection Range: |
7.8 pg/mL - 500 pg/mL |
| Minimum Detection Limit: |
7.8 pg/mL |
| Sample Type: |
Plasma, serum, tissue homogenate |
| Analytical Method: |
Quantitative |
| Sensitivity: |
4.6 pg/mL |
| Components: |
Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Sample/standard dilution buffer
Assay diluent
Biotin-labeled antibody (concentrated)
HRP-streptavidin (HRP-SA)
Biotin system (BS)
BS dilution buffer
TMB substrate
Stop solution
Wash buffer (25 x concentrate)
Instruction manual |
| Application: |
ELISA |
| Sample Volume: |
50 μL |
| Plate: |
Pre-coated |
| Specificity: |
This assay has high sensitivity and excellent specificity for detection of IL-12 p70. |
| Purpose: |
For quantitative detection of IL-12 p70 in serum, plasma, tissue homogenates and other biological fluids. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
For unopened kit: All reagents should be stored according to the labels on the vials. The standard and 96-well strip plate should be stored at - 20°C upon receipt, while the other reagents should be stored at 4°C.
For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper. |
| Expiry Date: |
6 months |
| Alternative Name: |
Interleukin 12 p70 |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
IL-12 (interleukin 12) is a heterodimeric cytokine made up of a 35-kDa light chain (p35 or IL-12A) and a 40-kDa heavy chain (p40 or IL-12B). The p35 gene is located on chromosome 3 in humans and chromosome 6 in mice, while the p40 gene is on chromosome 5 in humans and chromosome 11 in mice. The IL-12 receptor, which binds IL-12, consists of two subunits, IL-12RB1 (CD212) and IL-12RB2. IL-12 is a pro-inflammatory cytokine that promotes interferon-γ (IFN-γ) production, and T helper 1 (TH1) cell differentiation, and links innate and adaptive immunity. It is produced by dendritic cells and phagocytes in response to pathogens. The production of IL-12 is regulated by gene expression mechanisms, Toll-like receptor (TLR) patterns, and interactions between different dendritic cell subsets involving cytokines like IL-10 and type I IFN. |
