| Target: |
IL-12 (Interleukin 12) |
| Protein Type: |
Recombinant |
| Origin: |
Human |
| Source: |
HEK-293 cells |
| Purification tag / Conjugate: |
This IL12 protein is labelled with His-Avi Tag. |
| Sequence: |
Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A) |
| Purity: |
> 95% as determined by Tris-Bis PAGE, > 95% as determined by SEC-HPLC. |
| Endotoxin Level: |
Less than 1EU per μg by the LAL method. |
| Sterility: |
0.22 μm filtered |
| Format: |
Lyophilized |
| Reconstitution: |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Buffer: |
Lyophilized from 0.22 μm filtered solution in PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Handling Advice: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Storage: |
4°C, - 80°C |
| Storage Comment: |
Reconstituted protein stable at - 80°C for 12 months, 4°C for 1 week. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL-12 |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
Interleukin 12 (IL-12) plays a pivotal role as a versatile cytokine in coordinating the body's Th1-type immune response against cancer. Cancer is typified by uncontrolled cellular proliferation in particular areas of the body. |
| Molecular Weight: |
34.7 (IL - 12B) kDa / 25.5 (IL - 12A) kDa. Due to glycosylation, the protein migrates to 40 - 45 kDa based on Tris-Bis PAGE result. |
