| Target: |
IL-12 (Interleukin 12) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
C-term, extracellular domain |
| Origin: |
Human |
| Source: |
HEK-293T cells |
| Specificity: |
DNA sequence encoding extracellular domain of Human IL-12B fused to a C-terminal polyHis tag was expressed in HEK cells. |
| Characteristics: |
Recombinant human interleukin-12B p40 subunit is a monomer consisting of 306 amino acid residue and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE. |
| Purity: |
> 95%, as determined by SDS-PAGE |
| Endotoxin Level: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Sterility: |
0.2 μm filtered |
| Format: |
Lyophilized |
| Reconstitution: |
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Buffer: |
PBS solution pH7.4. |
| Handling Advice: |
Avoid repeated freeze/thaw cycles. |
| Storage: |
- 20°C |
| Storage Comment: |
The lyophilized antibody is stable for at least 1 year from date of receipt at - 20°C. Upon reconstitution, this antibody can be stored in working aliquots at - 8°C for one month, or at - 20°C for six months without detectable loss of activity. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL-12 |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
IL-6 emanates from a variety of cell types, primarily activated monocytes, fibroblasts, and endothelial cells in vivo. Upon stimulation, an array of other cells such as macrophages, T-cells, B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also engage in IL6 production. This cytokine belongs to a family that encompasses LIF, CNTF, Oncostatin M, IL11, and CT-1, all of which prompt the hepatic expression of acute phase proteins. The IL6 receptor is present on T-cells, mitogen-activated B-cells, peripheral monocytes, and certain macrophage- and B-cell derived tumor cells, yet it remains absent in quiescent B-cells but present in quiescent T-cells. Structurally, the IL6 receptor is a heavily glycosylated protein weighing 80 kDa and spanning 449 amino acids, identified as CD126. |
