| Target: |
IL-12 (Interleukin 12) |
| Biological Activity: |
Active |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 23-328 |
| Origin: |
Human |
| Source: |
HEK-293 cells |
| Purification tag / Conjugate: |
This IL12 protein is labelled with His tag, DYKDDDDK Tag. |
| Sequence: |
AA 23-328 |
| Characteristics: |
The heterodimer human IL-12B & IL-12A is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with his tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The predicted N-terminus is Ile 23 (IL12B) and Arg 23 (IL12A). The reducing (R) heterodimer protein migrates as 33-47 kDa due to glyosylation. |
| Purity: |
> 90% as determined by SDS-PAGE. |
| Endotoxin Level: |
Less than 1.0 EU per μg by the LAL method. |
| Sterility: |
0.22 μm filtered |
| Format: |
Lyophilized |
| Buffer: |
PBS, pH7.4 |
| Handling Advice: |
Please avoid repeated freeze-thaw cycles. |
| Storage: |
- 20°C |
| Storage Comment: |
No activity loss was observed after storage at: In lyophilized state for 1 year (4°C), After reconstitution under sterile conditions for 3 months (- 70°C). |
| Alternative Name: |
IL-12 |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
Interleukin 12 (IL-12), also referred to as p70, is a cytokine naturally synthesized by dendritic cells, macrophages, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. It exists as a heterodimeric structure comprising IL-12A (p35) and IL-12B (p40) subunits. IL-12 primarily facilitates the differentiation of naive T cells into Th1 cells and is recognized as a potent T cell-stimulating factor, promoting T cell growth and functionality. Additionally, it induces the production of IFN-γ and TNF-α from T cells and NK cells, while attenuating IL-4-mediated suppression of IFN-γ. IL-12 significantly contributes to the activities of natural killer cells and T lymphocytes. Moreover, it exhibits anti-angiogenic properties, impeding the formation of new blood vessels. |
| Molecular Weight: |
36.6 kDa (IL12B) and 23.8 kDa (IL12A) |
