| Target: |
IL-12 (Interleukin 12) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 23-328, AA 57-253 |
| Origin: |
Human |
| Source: |
CHO cells |
| Purification tag / Conjugate: |
This IL12 protein is labelled with Fc Tag. |
| Specificity: |
The human IL-12 complex composed of the p40 subunit (aa 23-328) and the p35 subunit (aa 57-253) is fused through a polypeptide linker to the Fc region of human IgG1. |
| Purpose: |
IL-12 (human):Fc (human) (rec.) |
| Characteristics: |
Protein. The human IL-12 complex composed of the p40 subunit (aa 23-328) and the p35 subunit (aa 57-253) is fused through a polypeptide linker to the Fc region of human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Interleukin-12 (IL-12), also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular immunity and as an antitumor and anti viral agent. |
| Purity: |
> 98% (SDS-PAGE) |
| Endotoxin Level: |
< 0.06 EU/μg protein |
| Format: |
Lyophilized |
| Concentration: |
Lot specific |
| Buffer: |
Lyophilized from 0.2 μm-filtered solution in PBS. |
| Handling Advice: |
Avoid freeze/thaw cycles. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Short term storage: + 4°C.
Long term storage: -20°C.
Use & Stability: stable for at least 1 year after receipt when stored at - 20°C. Working aliquots are stable for up to 3 months when stored at - 20°C. |
| Alternative Name: |
IL-12 |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
Interleukin-12 (IL-12), also recognized as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), constitutes a heterodimeric pleiotropic cytokine comprising a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Originating from macrophages and B lymphocytes, IL-12 manifests diverse impacts on T cells and natural killer (NK) cells. Its repertoire of activities encompasses the elicitation of IFN-gamma and TNF in both resting and activated T and NK cells, augmentation of cytotoxic activity in resting NK and T cells, stimulation of resting T cell proliferation alongside a comitogen, and fostering NK cell proliferation. Current scientific findings underscore IL-12's pivotal role as a mediator of cellular immunity, driving the differentiation of Th1 cells from precursor T helper cells. Given its multifaceted functionalities, IL-12 holds promise as a potential therapeutic agent, serving as a vaccine adjuvant to bolster cellular immunity and as an agent combating tumors and viral infections. |
