| Target: |
IL-6 (Interleukin 6) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 21-207 |
| Origin: |
Dog |
| Source: |
Baculovirus infected insect cells |
| Purification tag / Conjugate: |
This IL-6 protein is labelled with His tag. |
| Application: |
SDS-PAGE (SDS) |
| Sequence: |
FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEC VKHTTIHLIL RSLEDFLQFS LRAVRIMLEH HHHHH |
| Purity: |
> 95% by SDS - PAGE |
| Endotoxin Level: |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
| Format: |
Liquid |
| Concentration: |
0.5 mg/mL |
| Buffer: |
Liquid. In phosphate buffered saline (pH7.4) containing 10% glycerol. |
| Handling Advice: |
Avoid repeated freezing and thawing cycles. |
| Storage: |
4°C, - 20°C, - 80°C |
| Storage Comment: |
Can be stored at + 4°C short term (1-2 weeks). For long term storage, aliquot and store at - 20°C or - 70°C. |
| Alternative Name: |
IL6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
Interleukin-6 (IL-6) is a cytokine that undergoes phosphorylation and glycosylation variations. Produced by T cells and macrophages, it plays a vital role in triggering immune responses during infections and following injuries like burns, which induce inflammation. Interestingly, IL-6 also possesses anti-inflammatory properties. Recombinant canine IL-6, with a His-tag fused at the C-terminus, was produced in insect cells and purified using conventional chromatography methods. |
| Molecular Weight: |
22.0 kDa (195aa) 18 - 28 kDa (SDS-PAGE under reducing conditions) |
