| Dulbecco's Modified Eagle Medium (DMEM), available as a 10-liter powder form with a pH range of 5.9 to 6.3, is designed with high glucose, L-glutamine, and phenol red, offering a shelf life of 36 months. It maintains an osmolality of 240 to 280 mOsm/kg, suitable for mammalian cell culture.
DMEM serves as a widely utilized basal medium supporting the growth of various mammalian cell types. These include primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, and cell lines like HeLa, 293, Cos-7, and PC-12. Different formulations of DMEM are available to meet diverse cell culture requirements, ensuring optimal growth conditions.
DMEM distinguishes itself with a higher concentration of amino acids and vitamins compared to Eagle's Minimal Essential Medium. Originally formulated with low glucose (1 g/L) and sodium pyruvate, DMEM is commonly used with increased glucose levels, optionally with or without sodium pyruvate, adapting to specific experimental needs in cell biology |
