| Synonym(s): |
(±)-Dithiothreitol, rac-Dithiothreitol; Dithiothreitol; threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT |
| Formula: |
HSCH2CH(OH)CH(OH)CH2SH |
| Description: |
Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than βME, hence efficient in lower concentrations. |
| Application: |
General analytical |
| Form: |
Powder |
| Storage: |
2-8°C |
| Assay: |
≥ 98% (HPLC)
≥ 99.0% (titration) |
| Molecular Weight: |
154.25 |
| CAS Number: |
3483-12-3 |
| EC Number: |
222-468-7 |
| MDL Number: |
MFCD00004877 |
| PubChem Substance ID: |
24893251 |
| Color: |
White |
| mp: |
41-44°C (lit.) |
| Solubility: |
H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow. |
| SMILES String: |
O[C@H](CS)[C@H](O)CS |
| InChI: |
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1 |
| InChI key: |
VHJLVAABSRFDPM-QWWZWVQMSA-N |
| Quality Level: |
200 |
