| This solution contains 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin. Penicillin and streptomycin are utilized to prevent bacterial contamination in cell cultures due to their effective combined action against both gram-positive and gram-negative bacteria. Penicillin, originally purified from the fungi Penicillium, interferes directly with bacterial cell wall turnover and indirectly triggers the release of enzymes that further modify the cell wall. Streptomycin, originally purified from Streptomyces griseus, functions by binding to the 30S subunit of the bacterial ribosome, thereby inhibiting protein synthesis and causing death in susceptible bacteria. |
