| Description: |
Akt, also known as PKB or Rac, is pivotal in regulating cell survival and apoptosis. This protein kinase becomes active in response to insulin, as well as a variety of growth and survival factors, operating within a pathway sensitive to wortmannin that involves PI3 kinase. Akt's activation involves binding to phospholipids and phosphorylation at Thr308 in its activation loop by PDK1, as well as phosphorylation at Ser473 within the carboxy terminus. |
| Application: |
Western blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (paraffin) 1:150 - 1:600
Immunofluorescence (immunocytochemistry) 1:200 - 1:800
Flow cytometry (fixed/permeabilized) 1:100 - 1:400 |
| Storage: |
Supplied in 10 mM sodium HEPES (pH7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Molecular Weight: |
60 kDa |
| Antibody Source: |
Rabbit |
| Isotype: |
IgG |
| Reactivity: |
H M R Mk Dm |
| Sensitivity: |
Endogenous |
