| Description: |
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility. |
| Application: |
Western Blotting 1:1000
Simple Western 1:10 - 1:50
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Frozen) 1:100 - 1:400
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400 |
| Storage: |
Supplied in 10 mM sodium HEPES (pH7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Molecular Weight: |
45 kDa |
| Antibody Source: |
Rabbit |
| Specificity: |
β-Actin (13E5) rabbit mAb detects endogenous levels of total β-actin protein. Depsite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, β-Actin (13E5) rabbit mAb does not cross-react with cytoplasmic γ-actin, or any other actin isoforms. |
| Isotype: |
IgG |
| Reactivity: |
H M R Mk B Pg |
