| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Reactivity: |
Human |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
Flow cytometry (FACS), immunofluorescence (IF), neutralization (Neut) |
| Characteristics: |
This antibody neutralises human TNFa mediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of hTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. |
| Purification: |
Protein G affinity chromatography |
| Immunogen: |
Recombinant human protein was used as the immunogen for the TNF-alpha antibody. |
| Clone: |
J2D10 |
| Isotype: |
IgG1 kappa |
| Concentration: |
1 mg/mL |
| Buffer: |
1 mg/mL in 1X PBS, BSA free, sodium azide free. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store the TNF-alpha antibody at 2 - 8°C (with azide) or aliquot and store at - 20°C or colder (without azide). |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
This antibody blocks the harmful effects of human TNF alpha on L929 cells and inhibits tumor growth in mice. It also protects mice from the harmful effects of human TNF alpha. TNF alpha, a protein produced by stimulated macrophages, is associated with tissue damage and shock in certain conditions. It functions by causing cell death in some cancer cells and can be toxic to vascular endothelial cells. TNF alpha shares receptors and actions with another protein, TNF beta (lymphotoxin). Its effects include stimulating cell growth, activating certain immune cells and bone cells, and promoting the production of inflammatory substances. |
