| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 16.4 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
| AA Sequence: |
MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rRtbFGF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
FGF-2, HBGF-2 |
| Background: |
Rat bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Discovered in pituitary extracts in 1973, it was shown to stimulate fibroblast proliferation. Subsequent separation at different pHlevels yielded two variants, termed “acidic” (FGF-1) and “basic” (FGF-2) fibroblast growth factors. Rat bFGF shares 96% amino acid sequence identity with human bFGF and 98% with mouse bFGF. Heparin or heparan sulfate proteoglycan enhances its receptor affinity. bFGF is crucial for cell survival, division, angiogenesis, differentiation, and migration, and is involved in embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. It is also a key component in certain cell culture media, such as those for human embryonic stem cells and serum-free systems. |
