| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 12.4 kDa, a single non-glycosylated polypeptide chain containing 108 amino acids. |
| AA Sequence: |
SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 130 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuMEC/CCL28 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
CCK1 |
| Background: |
CCL28, a member of the CC family of chemokines, is also known as mucosae-associated epithelial chemokine (MEC). The CCL28 gene is located on chromosome 5 in humans, and its mRNA is prominently expressed by epithelial cells in various tissues, including the colon, salivary gland, mammary gland, trachea, and rectum, under normal and pathological conditions. The mature forms of human and mouse CCL28 share 83% sequence identity at the amino acid level. CCL28 signals through receptors CCR3 and CCR10, functioning as a chemotactic factor for resting CD4 and CD8 T-cells, as well as eosinophils. |
